- Whenever Marlene is mad at the penguins, she goes and yells at them down into their periscope e.g. Otter Things Have Happened and Truth Ache.
- She has a tendency to unexpectedly pop up.
- In Haunted Habitat, it was revealed that she has a combination of Phasmophobia (fear of ghosts) and Phonophobia (fear of loud voices).
- She was afraid of the outdoors. In Otter Gone Wild and Badger Pride, she went wild. Though in some episodes she has managed to stay normal e.g. Roomies and Work Order.
- She has good hygiene.
- If she is put in dangerous or stressful situation's, she shows strength and agility.
- She also knows a lot when it comes to romance (Monkey Love, Love Takes Flightless).
- She likes Pop Super Stars and pretends to have imaginary concerts every night as seen in Truth Ache.
- There is a picture of her with green eyes (along with the shorts), but she has brown eyes in all the episodes.
- She went on a date with Fred in Otter Things Have Happened, but broke up with him.
- She was believed to have cooties in Operation: Cooties but it was actually poison ivy.
- Her right foot is distinctive. If anything happened, her mother would recognize it (Badger Pride).
- She seemed to be Claustrophobic in The Big Squeeze.
- She is more verbal than physical.
- On some penguin operations, Skipper needs someone their size to help and Marlene is the right "guy" for the job (The Otter Woman, e.g. help getting sewer water and judge a burping contest).
- Marlene can belly slide like The Penguins, this is shown quickly in the episode Byte-Sized where she and the penguins slid pass a sign post to rescue Barry also in real life you can see them belly slide on snowy hills and icy lakes and ponds. (Note: She has belly slided in Roomies before.)
- She likes to sunbathe.
- As she is the main female animal in the zoo, there are many fan shippings: with Skipper, Private, Kowalski, Julien and Mort, with Skipper and Julien being the most popular. There is an article for each shipping in the Madagascar Fandom wikia.
- She has no longer became a monster being outside of the zoo in Littlefoot thanks to Kowalski's invention that separated her wild side and when it was befuddled by her, they rejoined together and Marlene has the power to control her side.
- She is mentioned in P.E.L.T. and Feline Fervor.
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