| "Character" | Movies | Animals | Zoo Workers |
 1. Purple Fly: (Various) -- Happy King Julien Day!, Roomies -- There are numerous purple flies around the zoo. Some were on the cake that Marlene made for King Julien, one made Rico sneeze when he was trying to stand still. and a few were on Marlene's hairbrush after Rhonda used it |
 2. Tick: Happy King Julien Day! -- These are the bugs that were on Mort and on the cake that Marlene made for King Julien |
 3. Caterpillar 1: Happy King Julien Day! -- These are the bugs that were on Mort and on the cake that Marlene made for King Julien on King Julien Day |
 4. Beetle: Happy King Julien Day! -- These are the bugs that were on Mort and on the cake that Marlene made for King Julien on King Julien Day |
 5. Caterpillar 2: Happy King Julien Day! -- These are the bugs that were on Mort and on the cake that Marlene made for King Julien on King Julien Day |
 6. Dogs: Monkey Love (mentioned) -- The dogs attacked the chimpanzees when King Julien made the mistake of ordering them to be released instead of pretty doves |
  7. Male Duck: Misfortune Cookie (Voiced by: Kevin Michael Richardson) -- This brown male duck accidentally landed on Rico's head |
 8. Possum 1: Mask of the Raccoon (lives in Central Park) (Voiced by: Kari Wahlgren) -- One of the Possums that lives in the park outside of the zoo and scavenges for food |
 9. Possum 2: Mask of the Raccoon (lives in central Park) -- One of the Possums that lives in the park outside of the zoo and scavenges for food |
 10. Butterfly: Otter Gone Wild -- Marlene saw this butterfly in the park and tried to catch it |
 11. Firefly: What Goes Around -- Rico coughed up this firefly while looking for a warm feeling inside his stomach |
 12. Crab: Go Fish (Voiced by: n/a) -- Seen on a vacation slide on top of Kowalski who was buried in sand |
 13. Great White Shark: Go Fish (Voiced by: n/a) -- Seen on a vacation slide on top of Kowalski who was buried in sand |
| "Character" | Movies | Animals | Zoo Workers |