| "Character" | Movies | Animals | Zoo Workers |
 1. "Bob": Happy King Julien Day! (Voiced by: n/a) -- King Julien named a paper horse pinata "Bob". He has candy guts |
 2. Space Squid: Lemur See, Lemur Do -- The Space Squid was first seen on the cover of a comic book that Skipper had. Only Skipper was shown to believe that they actual existed. It was later revealed to be a real creature when one of its tentacles abducted the LEM-R robot on national TV. |
 3. Ghost Sheep: Two Feet High and Rising (Voiced by: Jeff Bennett) -- Mort dreams of these sheep jumping over a fence |
 4. Tasty Fish: Tangled in the Web (Voiced by: Kevin Michael Richardson) -- The fish that Private is trying not to eat starts talking to him saying how tasty it is. |
 5. Tourist: Gone in a Flash (Voiced by: Kevin Michael Richardson) -- Lost his camera and went back to the zoo to try to find it in the episode "Gone in a Flash". He is slightly balding and wears glasses and a cargo vest. He has another camera around his neck |
 6. Commercial: Launchtime (Voiced by: Kevin Michael Richardson) -- There was an ad for a $49.99 fish on the penguins TV. It was a "once in a lifetime opportunity" |
 7. Toy Factory Workers: Operation: Plush & Cover (Voiced by: n/a) -- Two of these guys appeared loading up a truck full of bad Mort dolls, a few more appeared back at the toy factory. They wear green uniforms with matching hats |
 8. CW Smith: Operation: Plush & Cover (Voiced by: n/a) -- The toy factory worker who signed for the delivery of bad Mort dolls at the factory when they came back. Was knocked unconscious by Rico. He has a patch on his arm that says "box boy" |
 9. Auto Insurance Salesman: Penguiner Takes All (Voiced by: Kevin Michael Richardson -- Was on a TV commercial asking if you paid too much for your auto insurance) |
 10. Annoying Crowd Guy: Tangled in the Web (Voiced by: Jeff Bennett) -- This guy made it a point to announce "Slippy's" debut to everyone that was already watching |
 11. Narrator for "Carnage of the Penguins" DVD documentary: All Choked Up (Voiced by: Jeff Bennett) -- The narrator for the animal documentary about penguins being killed in Antarctica |
 12. Boy with Popcorn: Popcorn Panic (Voiced by: Tara Strong) -- Was caught by Alice feeding popcorn to the animals |
 13. Fish Truck Driver: Go Fish -- The guy who drives the Fish E truck |
 14. Crane Operator: Roomies -- Works on the Manhattan shipping docks as the crane operator. Was knocked out by Kowalski |
 15. Chinese take out guy: Misfortune Cookie (Voiced by: Jeff Bennett) - Took the order for Chinese food over a payphone |
 16. Chinese food Delivery Guy: Misfortune Cookie (Voiced by: Danny Jacobs) -- Delivered Chinese food to the zoo animals |
 17. Balloon Holder: Skorca! -- There were a bunch of these guys holding the ropes for the giant balloon parade floats. Joey the kangaroo did battle with them |
 18. Smart Kid: Miss Understanding (Voiced by: Kari Wahlgren) -- A kid on a field trip to the zoo who knows way too much about the penguins and has to prove it by stating off facts to Alice |
 19. Radio Control Guy 1: Otter Gone Wild (Voiced by: Jeff Bennett) -- Flies remote control airplanes at the park. Was knocked out by the penguins |
 20. Radio Control Guy 2: Otter Gone Wild -- Flies remote control airplanes at the park. Was knocked out by the penguins |
 21. Little Girl: What Goes Around (Voiced by: Ariel Winter) -- This little girl lost her doll at the zoo but eventually got it back thanks to the penguins |
 22. Girl's Mom: What Goes Around (Voiced by: n/a) -- The mother of the little girl lost her doll at the zoo |
 23. Delivery Truck Driver: Monkey Love (Voiced by: James Patrick Stuart) -- Drives the delivery truck with live animals from the Hoboken zoo |
 24. Doorman: What Goes Around (Voiced by: James Patrick Stuart) -- Works as the front door man for an apartment building. Likes checking out pretty ladies. Was knocked out by the penguins |
 25. Soap Opera Doctor: I Was a Penguin Zombie -- This doctor was on a soap opera on TV |
 26. Soap Opera Nurse: I Was a Penguin Zombie (Voiced by: Mary Scheer) -- This is the doctors nurse assistant who was on a soap opera on TV |
 27. Apartment Security Guy: What Goes Around (Voiced by: John DiMaggio) -- Operates the closed circuit monitors for the apartment building security. Called animal control on the penguins. We only get to see his arm |
 28. School Bus Driver: Sting Operation -- The guy who drives the school bus full of children to the zoo |
  29. Kids -- The kid with the hat is named Vic (voiced by Tara Strong might be same kid as #12 and #18). The other one is his cousin (voiced by Grey DeLisle). They were seen in the episode Gator Watch |
 30. Screaming Lady in Park. She was seen in Gator Watch (voiced by: Grey DeLisle) |
  31. Graveyard 8 Driver: Concrete Jungle Survival (voiced by John DiMaggio) |
| "Character" | Movies | Animals | Zoo Workers |